The Expression and Correlation of DNA ... - Cancer Research Center

[Abstract]

Objective: In our study, we detect Fragile histidine triad gene ?FHIT? and human DNA mismatch repair genes ?hMMR? hMSH2 in invasive cervical cancer?cervical intraepithelial neoplasia ?CIN? and normal cervixs. To identify the expression of FHIT and hMSH2 in cervical cancer and their possible relationship in the process of cervical cancer?s occurrence and development, aiming to try to provide a new angle of view in its measure.Method: In our study, we selected 35 patients who had invasive cervical cancer?10 patients who had cervical intraepithelial neoplasia ?CIN? and we also included 30 patients who had undergone hysterectomy for begnign uterine pathology, with histologically normal cervix, as a control group. For each patient, we use immunohistochemical analysis to detect the expression of FHIT and hMSH2 genes,furthermore, we analysized the clinical datas to indentify the correlation between the two genes and clinicalcopathological feature.Result:1. Expression of hMSH2: The hMSH2 protein expression was mainly found in nuclear. The positive rate in carcinoma, CIN and normal cervix were 60.0 % ?21/35??70.0% ?7/10? and 30.0% ?9/30?, respectively, the difference was significant ???0.05?. The abnormal expression of hMSH2 had something to do with histological grade ???0.05?, but has no significant difference among other clinicalcopathological features ???0.05?.2. Expression of FHIT?The expression of FHIT protein was in cytoplasmic. The FHIT expression in normal tissue showed to be normal or even positive, no cases occurred descending or short, abnormal one reached 0.0% ?0/30?; in the carcinoma ??and CIN specimens, 22 cases and 4 cases showed the expression descending or short, abnormal one reached 62.9%?22/35? and 40%?4/10?, respectively, difference was significant ???0.05?; The descending and short expression of FHIT had something to do with histological grade ???0.05?. There was no significant difference among other clinicalcopathological features, such as smoking, age, FIGO stage and so on ???0.05?.3. In the carcinoma specimens, there is no significant correlations between the two genes (r_s=-0.097,??0.05).Conclusion:1. The normal expression of human DNA mismatch repair gene ?hMMR? plays a key role in maintaining the genetic stability. The positive rate of hMSH2 protein in CIN?carcinoma,and normal cervix were decreasing by turns, suggesting that the expression of hMSH2 is associated with carcinogenesis of cervical cancer, and its high expression may be a potential marker of CIN and cervical cancer.2. The ascending or descending of FH1T expression plays an important role, which might influence the prognosis of cervical carcinoma.3. The expression of FHIT protein was not associated with the expression of hMSH2 protein significantly (r_s??0.097,??0.05).

Title: The Expression and Correlation of DNA Mismatch Repair Gene hMSH2 and Fragile Histidine Triad FHIT in Cervical Carcinoma

Category: Ovarian Cancer

Filename: The Expression and Correlation of DNA Mismatch Repair Gene hMSH2 and Fragile Histidine Triad FHIT in Cervical Carcinoma.pdf

Pages: 189

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